Product name
Canine IgE ELISA Kit
Detection method: colorimetric
Example type
Serum, Plasma, Other biological fluids
Essay type: sandwich (quantitative)
Sensitivity: 2.2897ng/mL
Rank: 6.25ng/mL – 400ng/mL
Test duration: Standard multi-step assay
Species reactivity
Reacts with: Dog
Product description
The Abcam IgE Dog ELISA Kit is a highly sensitive two-site enzyme-linked immunoassay (ELISA) for the quantitative measurement of IgE in dog serum and plasma samples. In this assay, IgE present in samples reacts with anti-IgE antibodies that have been adsorbed on the surface of polystyrene microtiter wells. After removing unbound proteins by washing, horseradish peroxidase (HRP)-conjugated anti-IgE antibodies are added. These enzyme-labeled antibodies form complexes with previously bound IgE.
After another wash step, the immunosorbent-bound enzyme is analyzed by the addition of a chromogenic substrate, 3,3′,5,5′-tetramethylbenzidine (TMB). The amount of bound enzyme varies directly with the concentration of IgE in the analyzed sample; therefore, the absorbance, at 450 nm, is a measure of the concentration of IgE in the test sample. The amount of IgE in the test sample can be interpolated from the standard curve constructed from the standards and corrected for sample dilution.
Platform: microplate
Properties
Storage instructions
Store at +4°C. Check the protocols.
Relevance
IgE is the class of antibodies produced in the lungs, skin, and mucous membranes. It can protect against parasite invasion, but is a major factor in allergic reactions. Antigen-specific IgE interacts with mast cells and eosinophils, triggering the release of histamine, leukotrienes, and other substances that lead to the itching, sneezing, and congestion of allergies, and the life-threatening shortness of breath of asthma and anaphylactic shock .
Cell localization: secreted
Alternative names
- Ig epsilon chain region C
- IgE
- IGHE
- immunoglobulin heavy constant epsilon