Native proteins are proteins that are in their folded and/or assembled form and are fully functional. They are not altered by denaturing agents, such as heat, chemical or enzymatic action or the demands of extraction. The native state of a biomolecule can possess all four levels of biomolecular structure, with secondary to quaternary structure formed from weak interactions along the covalently bonded backbone.
Fitzgerald Industries offers a wide range of native protein antigens, extracted unmodified from animal, cellular or serum sources. We supply native proteins for use in ELISA, Western Blot, immunohistochemistry, functional assays, lateral flow, and more.
We ship thousands of human, animal, viral, and bacterial proteins in their native state in a variety of purity levels or conjugated with labels including biotin, fluorescein isothiocyanate (FITC), horseradish peroxidase (HRP), alkaline phosphate, and others.
Our native proteins are the ideal choice for researchers studying cell biology and growth factors, protein genetics and modification, cancer treatments, cardiac markers, signal transduction, nutrition and metabolism, enzyme action, and much more.
Some of our most popular native proteins include:
- CA 125 protein
CA-125 (MUC16) is gaining importance as a tumour marker or biomarker that may be elevated in the blood of some patients with specific types of cancer or other benign conditions. Our purified native CA 125 protein is purified from human ascitic fluids and is >95% pure. It can be used in a number of applications as a positive control, such as Western blotting, ELISA, and Lateral Flow. This CA125 protein is partially purified from a human ovarian adenocarcinoma cell line and is also an experimental control.
- CEA protein
The carcinoembryonic antigen (CEA) is involved in cell adhesion. It normally occurs during fetal development, but CEA production stops before birth. Therefore, it is not usually present in the blood of healthy adults, making it a tumour marker in cancer research. This CEA protein is purified >80% by the human colon carcinoma immunoaffinity column. This CEA protein is ideal for ELISA assays, while this highly purified CEA protein can be used as a control in a wide range of assays.
- CA 15-3 protein
Another tumour marker, CA 15-3, has shown high specificity for breast tumours. Detection of breast cancer recurrence by the CA 15-3 assay may precede confirmation by radiological detection methods by 1 to 9 months. Although many other biomarkers related to breast cancer have been discovered and clinically investigated, no protein has been shown to have greater specificity or sensitivity than breast tumour antigen CA 15-3. This high purity CA 15-3 protein can be used in IHC and ELISA as a positive control, while our CA 15-3 protein is also ideal for use as a control in assay development.
- Myoglobin protein
Myoglobin is released into the bloodstream after muscle injury, making it an ideal marker for cardiac injury. This myoglobin protein is 99% pure and comes from human heart tissue. This myoglobin protein has been validated in latex immunoturbidimetric assays and is available in bulk.